Autofluorescence and Metabotyping of Soybean Varieties Using Confocal Laser Microscopy and High-Resolution Mass Spectrometric Approaches

Razgonova M. P., Navaz M. A., Butovets E. S., Lukyanchuk L. M., Chunikhina O. A., ERCİŞLİ S., ...Daha Fazla

Plants, cilt.14, sa.13, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 13
  • Basım Tarihi: 2025
  • Doi Numarası: 10.3390/plants14131995
  • Dergi Adı: Plants
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Glycine max(L.) Merr, laser microscopy, polyphenolic compounds, soybean, tandem mass spectrometry
  • Atatürk Üniversitesi Adresli: Evet

Özet

This research examines a detailed metabolomic and comparative analysis of bioactive substances of soybean varieties: “Primorskaya-4”, “Primorskaya-86”, “Primorskaya-96”, “Locus”, “Sphere”, “Breeze”, “Namul”, and “Musson” by the laser confocal microscope CLSM 800 and the mass spectrometry of bioactive compounds by tandem mass spectrometry. The laser microscopy allowed us to clarify in detail the spatial arrangement of phenolic acids, flavonols, and anthocyanin contents in soybeans. Research has convincingly shown that the polyphenolic content of soybeans, and, in particular, the anthocyanins, are spatially localized mainly in the seed coat of soybeans. Tandem mass spectrometry was used to identify chemical constituents in soybean extracts. The results of initial studies revealed the presence of one hundred and fourteen compounds; sixty-nine of the target analytes were tentatively identified as compounds from polyphenol groups.