Assessment of 3-Amino-Benzoic Acid Methyl Ester Derivatives as Glutathione S-Transferase and Glutathione Reductase Inhibitor: Supported by Molecular Docking Studies

Korkmaz I. N., GÜLLER P., Kalın R., ÖZDEMİR H.

ChemistrySelect, cilt.9, sa.26, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 26
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1002/slct.202401362
  • Dergi Adı: ChemistrySelect
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Anahtar Kelimeler: Glutathione reductase, Glutathione S-transferases, In vitro inhibition, Methyl 3-amino benzoates, Molecular docking
  • Atatürk Üniversitesi Adresli: Evet

Özet

GSTs catalyze detoxification reactions of harmful xenobiotics via conjugation with glutathione (GSH) while glutathione reductase (GR) is the sole enzyme that acts in the recovery reaction of GSH from oxidized glutathione (GSSG). In this study, in vitro inhibitory impacts of 3-amino-benzoic acid methyl ester compounds on GR and GST were investigated. For this firstly, GR and GST were obtained from human blood with specific activity of 6.26 EU/mg protein and 8.57 EU/mg protein respectively. Then, inhibition studies were performed. It was found that methyl 3-amino-5-chlorobenzoate had the highest inhibitory effect on hGR with Ki value of 0.524±0.109 μM) and methyl 3-amino-4-nitrobenzoate was the most effective inhibitor on hGST with Ki value of 37.05±4.487 μM. Besides, molecular docking analysis was used to estimate the binding energies of molecules. Methyl 3-amino-4-nitrobenzoate and methyl 3-amino-4-chlorobenzoate were predicted to have the highest binding affinity into GR and GST receptors respectively.