The Effect of Sodium Pertechnetate Human Carbonic Anhydrase I and II

ŞAHİN A., ŞENTÜRK M.

International Conference on Advances in Natural and Applied Sciences (ICANAS), Antalya, Türkiye, 18 - 21 Nisan 2017, cilt.1833 identifier identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 1833
  • Doi Numarası: 10.1063/1.4981758
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Atatürk Üniversitesi Adresli: Evet

Özet

The inhibitory effects of (NaTcO4)-Tc-99m (Sodium pertechnetate) on human erythrocyte carbonic anhydrase I and II activity were investigated. For this purpose, hCA I was initially purified 114,29-fold at a yield of 69,19 % and hCA II was initially purified 710,82-fold at a yield of 71,72 % using sepharose 4B-tyrosine-sulfanilamide affinity gel chromatography. The in vitro effect of this compound on hCA I and II isoenzyme were studied. It was detected in in vitro studies that the hCA I and II enzymes are inhibited due to (NaTcO4)-Tc-99m.