A combination of solid-phase extraction and dispersive solid-phase extraction effectively reduces the matrix interference in liquid chromatography-ultraviolet detection during pyraclostrobin analysis in perilla leaves

Farha W., Rahman M. M., Abd El-Aty A. M. A., Jung D., Kabir M. H., Choi J., ...More

BIOMEDICAL CHROMATOGRAPHY, vol.29, no.12, pp.1932-1936, 2015 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 29 Issue: 12
  • Publication Date: 2015
  • Doi Number: 10.1002/bmc.3523
  • Journal Name: BIOMEDICAL CHROMATOGRAPHY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1932-1936
  • Keywords: pyraclostrobin, perilla leaf, LC-UVD, interferences, SPE-d-SPE combination, TANDEM MASS-SPECTROMETRY, DIODE-ARRAY DETECTION, TIME-OF-FLIGHT, STROBILURIN FUNGICIDES, PESTICIDE-RESIDUES, GLOBAL UNCERTAINTY, DISSIPATION, VALIDATION, GRAPES, AZOXYSTROBIN
  • Ataturk University Affiliated: No

Abstract

Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid-phase extraction and dispersive solid-phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (R-2 = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06- 89.10% of the target compound was recovered with a relative standard deviation <4. The limits of detection and quantification were 0.0033 and 0.01 mg/kg, respectively. The method was successfully applied to field samples collected from two different areas at Gwangju and Muan. The decline in the resiudue concentrations was best ascribed to a first-order kinetic model with half-lives of 5.7 and 4.6 days. The variation between the patterns was attributed to humidity. Copyright (c) 2015 John Wiley & Sons, Ltd.