The effect of brimonidine and proparacaine on metabolic enzymes: Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase

ÇALIŞKAN B., Ozturk Kesebir A., Demir Y., AKYOL SALMAN İ.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.69, sa.1, ss.281-288, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/bab.2107
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.281-288
  • Anahtar Kelimeler: glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glutathione reductase, brimonidine, proparacaine
  • Atatürk Üniversitesi Adresli: Evet

Özet

Oxidative stress is to upregulate the pentose phosphate pathway (PPP). The PPP consists of two functional branches, glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconaste dehydrogenase (6PGD). Glutathione reductase (GR) has a significant role in catalyzing an oxidized glutathione form into a reduced form. The purpose of this study is to investigate the effects of brimonidine and proparacaine on the activity of 6PGD, G6PD, and GR enzymes purified from human erythrocytes. Brimonidine displayed considerable inhibition profile against G6PD with IC50 value and K-I constant of 29.93 +/- 3.56 and 48.46 +/- 0.66 mu M, respectively. On the other hand, proparacaine had no inhibitory effect against G6PD. K-I values were found to be 66.06 +/- 0.78 and 811.50 +/- 11.13 mu M for brimonidine and proparacaine, respectively, for 6PGD. K-I values were found to be 144.10 +/- 2.01 and 1,654.00 +/- 26.29 mu M for brimonidine and proparacaine, respectively, for GR. Herein, also in silico molecular docking studies were performed between drugs and enzymes.