Akademik Veri Yönetim Sistemi
2. BASKENT INTERNATIONAL CONFERENCE ON MULTIDISCIPLINARY STUDIES, Ankara, Türkiye, 24 Şubat 2022, cilt.1, ss.266-274
This study aims to investigate the polymorphism of Diacylglycerol acyltransferase1 (DGAT1) gene
locus in Tushin sheep raised at Atatürk University Food and Livestock Application and Research Center
Farm and to determine the distribution of genotype and allele frequencies of sheep in terms of related
genes. DGAT1/Alu1 genes polymorphism was defined by using the PCR-RFLP method in the DNAs
isolated from hair samples taken from 100 Tuj sheep used in this study. PCR- RFLP products were run
in an electrophoresis medium and the results were visualized as bands in an ultraviolet (UV) device. In
the study, distributions of the genotype and allele gene frequency of the population were calculated and
it was tested whether the populations comply with the Hardy-Weinberg (HW) genetic equilibrium test.
According to the Hardy-Weinberg genetic equilibrium test, it was observed that the distribution of
genotype frequencies was in balance (P>0,05) in the population studied. The CC, CT, and TT genotype
frequencies of the DGAT1 gene in the population were found to be 53%, 38%, and 9% for the Tuj sheep
breed, respectively. When the population was examined in terms of allele frequencies, it was defined
that the C allele and the T allele were 0.72 and 0.28 for the Tuj sheep, respectively. DGAT1 genotypes
were determined using the PCR-RFLP method from hair samples taken from Tushin sheep. It was observed that the distribution of the DGAT1 gene locus in the examined sheep breed population was in
balance according to the HW genetic equilibrium test. It has been defined the genotype and allele
frequencies determined in terms of DGAT1 gene polymorphism may be found to be sufficient to reveal
the genotype diversity of this breed.