Akademik Veri Yönetim Sistemi
2. Uluslararası ve 27. Ulusal Farmakoloji Kongresi / 2nd International and 27th National Pharmacology Congress , Antalya, Türkiye, 23 - 26 Kasım 2023, ss.134-135
Purpose: Galangin is a bioactive flavonoid found in honey and Alpinia officinarum. Our aim in this study is to try to explain the possible healing effect of galangin, which has a wide range of biological activities, in the L929 cell line scratch wound test model through TGF-β1. Material-Method: L929 cell line (ATCC, USA) was used in the study. Cells were seeded in DMEM medium containing 10% fetal bovine serum (FBS) following the routine thawing procedure. It was incubated for 48 hours at 37°C, 5% CO2 and 90% humidity. The number of proliferating cells was determined. It was seeded on a 96-well plate at 1 × 104 cells/well. In vitro cell viability was determined by MTT analysis by applying 7 doses of galangin (1, 10, 25, 50, 100, 250, 500 µM) at 24, 48 and 72 hours. In the study, a scratch wound test model was created as a wound healing model. According to the MTT results, it was decided to use galangin in doses of 10, 25, 50µM. Cells were seeded in a 6-well plate at 2 × 104 cells/well. After a full layer of L929 cells was formed in the well, a scratch was created vertically in each well with a sterile pipette tip. Photographs of the cells at 0, 12, 24 and 36 hours were taken with a Leica Inverted Microscope (Leica, DMIL LED). Percent wound healing was calculated. TGF-β1 amounts from the supernatant obtained at the 36th hour were measured by ELISA method and evaluated statistically. Results: It was observed that galangin at doses of 25 µM and 50 µM increased the wound healing and migration rate compared to the control group. It was observed that the amount of TGF-β1 decreased at 25 µM and 50 µM doses of galangin compared to the control (p<0.05). Conclusion: In the study, it was shown that galangin, a flavonoid, had significant effects on wound healing in the scratch wound model performed on the L929 fibroblast cell line. We think that this effect of galangin may occur by increasing fibroblast migration via TGF-β1. We believe that Galangin may be a new treatment option in wound healing if our study is supported by future studies. Keywords: Galangin, Scratch Wound Assay, Migration, TGF-β1