Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

Ferah Okkay I., Okkay U., Aydin I. C., Bayram C., Ertugrul M. S., Mendil A. S., ...Daha Fazla

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.29, sa.22, ss.33774-33784, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 22
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s11356-022-18626-z
  • Dergi Adı: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.33774-33784
  • Anahtar Kelimeler: Apoptosis, Centella asiatica, Cisplatin, Hepatotoxicity, Inflammation, Oxidative stress, TOTAL TRITERPENIC FRACTION, INDUCED LIVER-INJURY, HEPATIC-INJURY, MICROANGIOPATHY, MADECASSOSIDE, ANTIOXIDANT, TOXICITY, PATHWAY, RATS, MICE
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study was designed to investigate the protective effects of Centella asiatica (CA) on cisplatin-induced hepatotoxicity and to clarify the underlying mechanism by biochemical, molecular, immunohistochemical, and histopathological analyses. Rats were pre-treated with two doses of CA (100 and 200 mg/kg, p.o.) for 14 consecutive days. Then, on the 15th day, hepatotoxicity was induced by a single cisplatin injection (10 mg/kg i.p.). On the 18th day, the rats were euthanized. CA effectively alleviated cisplatin-induced hepatic injury via reduction in AST, ALT, and ALP enzymes and a decrease in oxidative stress (decreased MDA and ROS, and increased SOD, CAT, and GSH). CA also mitigated the inflammatory damage by the inhibition of TNF-alpha, IL-1 beta, and NF-kappa B. The liver expression of caspase-3 and Bax was downregulated, while Bcl-2 was upregulated. Moreover, immunohistochemical results confirmed the recovery with CA by downregulation of iNOS and 8-OHdG expression. These results showed that with its antioxidant, anti-inflammatory, and anti-apoptotic activities, CA could help alleviate the hepatotoxic effects of cisplatin chemotherapy.