Akademik Veri Yönetim Sistemi
3. International Environmental Chemistry, Antalya, Türkiye, 1 - 04 Kasım 2021, ss.16
Diabetes Mellitus is the commonest major metabolic disease with high mortality and morbidity risk in the
world which is characterized by hyperglycemia induces. Although the mechanism of trace elements on
diabetic patients has been explained as providing the activation of insulin receptor sites, being a cofactor or
component for enzymes in glucose metabolism, and acting as an antioxidant that increases insulin sensitivity,
the effect of each trace element has not yet been fully elucidated.1
Zinc and boron, which have important
roles in glucose and lipid metabolism, respectively, have important roles in the secretion, synthesis, and
storage of insulin. In addition, with the antioxidant properties of both trace elements, it can play an important
role in diabetes caused by oxidative stress.2, 3
The aim of this study was to determine and compare the serum zinc and boron levels in normal and diabetic
rats in order to understand the roles of both zinc and boron in diabetes. Type 1 diabetes was induced in rats
by administration intraperitoneally in a single dose of high dose streptozotocin (STZ) to the overnight fasted
rats. After three days of STZ administration, rats were considered as diabetic rats, if the fasting blood glucose
levels measured by Accu-Chek glucometer >300 mg/dL. Six rats, each with STZ-induced diabetic and control,
were included in the study. Rats were sacrificed under deep anesthesia, and later blood samples were taken
from the heart of rats. Inductively Coupled Plasma-Mass Spectrometry ICP-MS method, which is very elegant
procedure for evaluation the analysis of multiple elements at the same time, was used in this study to
determine the zinc and boron in serum. For serum sample preparation, nitric acid and hydrogen peroxide
were added to the serum samples and obtained solution were burned by using blood method in Milestone
ETHOS UP High-Performance Microwave Digestion System. After degradation, samples were diluted with
Milli-Q deionized water and analyzed by ICP-MS. The proposed ICP-MS method was validated. With this
purpose, calibration curve obtained by plotting calibration curves between peak areas and added
concentrations to be determine the method linearity. The equations for calibration curves were obtained in
10-500 ng/mL concentration range for both Zinc and Boron.
The method was found as precise with 3.25 RSD%, and accurate with ± 2.58 RE % for both elements. The
mean recovery % was determined as 101.4±5.46% for boron and 98.2±4.53 % for zinc. The limit of detection
(LOD) and limit of quantification (LOQ) values found as 2.14 ng/mL and 7.06 ng/mL for boron and 1.55 ng/mL
and 5.11 ng/mL for zinc, respectively. The both serum zinc and boron levels in the STZ-induced diabetic rats
were significantly lower than control rats (P < 0.01).