Fluorescence quenching of fluorescein with molecular oxygen in solution

Arik M., CELEBI N., ONGANER Y.

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, cilt.170, sa.2, ss.105-111, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 170 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.jphotochem.2004.07.004
  • Dergi Adı: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.105-111
  • Anahtar Kelimeler: fluorescein, molecular oxygen, dynamic quenching, bimolecular quenching rate constant
  • Atatürk Üniversitesi Adresli: Evet

Özet

The fluorescence quenching of fluorescein (FL) with molecular oxygen in alkali solution and in different solvents was investigated by steady-state absorption and fluorescence spectroscopy techniques. Fluorescence quantum yield (Phi(f)), radiative rate constant (k(r)), fluorescence lifetime (tau(f)), and non-radiative rate constant (k(nr)) values were determined. Stern-Volmer equation was employed to calculate bimolecular quenching rate constants (k(q)) and, then, Smoluchowski equation was used for the determination of diffusion-controlled rate constants (k(diff)) to compare with the experimental k(q) values. The temperature, quencher concentration, and solvent viscosity were experimental parameters to understand fluorescence quenching process of fluorescein with molecular oxygen in solution. Experimental findings indicated that the mechanism of fluorescence quenching is diffusive in nature. (C) 2004 Elsevier B.V. All rights reserved.