Akademik Veri Yönetim Sistemi
Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.22, sa.6, ss.929-936, 2016 (SCI-Expanded)
© 2016, Veteriner Fakultesi Dergisi. All rights reserved.The aim of this study was to investigate the effect of Taraxacum officinale extract (TOE) on gentamicin-induced reproductive damage in male rats. Totally, 24 male Sprague Dawley rats were divided into four groups: Group I (n=6); referred as control, physiological saline was intraperitoneally (IP) administered. Group II (n=6); referred as gentamicin (G), 80 mg/kg gentamicin sulphate (GS) was injected IP. Group III (n=6); referred as G + TOE150, 80 mg/kg GS and 150 mg/kg TOE was given IP. Group IV (n=6); referred as G+TOE200 (n=6), 80 mg/kg GS and 200 mg/kg TOE was administered IP. The treatment continued for consecutive 8 days. The cauda epididymal semen samples and testes tissues were collected. Routine semen examinations were performed and oxidative stress levels of testicular tissues were assayed. Reproductive organ weights [total testes weight (TTW) and total cauda epididymal weights (TCEW)] were recorded. GS administration significantly decreased sperm motility (P<0.01), glutathione peroxidase (GPx) activity (P<0.001) and glutathione (GSH) level (P<0.05), and it significantly increased tissue malondialdehyde (MDA) level (P<0.01) in comparison with the control group. However, a statistical increase in sperm motility of GT150 (P<0.01) group and in GPx activities of both GT150 and GT200 (P<0.01) groups as well as a statistical decrease in MDA levels of GT150 and GT200 (P<0.001) groups were determined when compared with the G group. In conclusion, short-term administration of GS causes lipid peroxidative damages in testes as well as decreases in sperm motility. However, TOE has a moderate ameliorative effect on sperm motility reductions, but marked improvement effect on lipid peroxidative testicular damages induced by GS.