Hsp-70 gene expression analyses in the different ages of rainbow trout Farklı yaşlardaki gökkuşaǧı alabalıklarında (Oncorhynchus mykiss) Hsp-70 geninin ekspresyon analizi

Alak G., Çiltaş A., Erdoğan O.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.16, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16
  • Basım Tarihi: 2010
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Atatürk Üniversitesi Adresli: Evet

Özet

The purpose of this study was to determinate the association between heat shock protein 70 (Hsp-70) expression and the age in rainbow trout. Total RNA was extracted with TRIzol Reagent (Life Technologies) and the first strand cDNA was synthesized using Super Script III Reverse Transcriptase. Gene amplification was done by PCR method and quantitative determination of gene expression was using beta actin gene as control. Consequently, we demonstrated that Hsp70 expression significantly increased the age.