Akademik Veri Yönetim Sistemi
HOCA AHMET YESEVİ 2. ULUSLARARASI BİLİMSEL ARAŞTIRMALAR KONGRESİ, Erzurum, Türkiye, 6 - 08 Aralık 2019, ss.272-277
Nowadays, the largest group of species and number of species living, insects (hexapods), both
ecologically and economically effective in human and animal populations. Ecological planning has
become the subject of endemic, bio-indicator, intestinal flora of suitable insects suitable for agricultural
production or harmful insects, and recently focused on environmentally friendly planning. As the flora
is the most important bacteria of the insect microbiota. These microorganisms play a positive or negative role in maintaining vital activities for insects. In our study, a sample of the insect species of
Enochrus quadripunctatus (Herbst, 1797) (Coleoptera: Hydrophilidae) was collected live from aquatic
environments and treated with 70% ethyl alcohol for 5 minutes to remove possible contaminant
microorganisms on the sample. The alcohol was then washed with sterile distilled water. After surface
sterilization, insects were knocked out with ether and the digestive tract was separated in a standard
aseptic conditions by dissection. Strains were made for identification of bacteria without waiting from
the separated intestinal sample. The bacteria were then identified on the by VITEK 2 (bioMérieux). On
the of the study, gram negative stained microorganisms were identified with VITEC2 Aeromonas
veroni, A. hydrophila / caviae and Pseudomonas aeroginosa. Pseudomonas auroginosa was found to
be pigment-free. In our study, two separate bacteria could not be identified with VITEK 2 device. With
this study, it is expected that aquatic microbiota will contribute to biotechnological research and
biological control methods in terms of agricultural and environmental research. For this reason,
hexapod species selected as an example in the study were examined from aquatic environments. Our
research is a preliminary study and more samples and advanced techniques are needed to determine
the microbiota of this insect group. We think that our study is guiding for such studies to be conducted
later