An enzymatic method for the determination of free fatty acids in serum/plasma

Kiziltunc A., Akcay F.

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, cilt.36, sa.2, ss.83-86, 1998 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 2
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1515/cclm.1998.015
  • Dergi Adı: CLINICAL CHEMISTRY AND LABORATORY MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.83-86
  • Atatürk Üniversitesi Adresli: Evet

Özet

Estimation of serum free fatty acids (FFA) in serum based on the formation of inorganic phosphate has been simplified by eliminating complex stages. The principle of the present method is based on breakdown of pyrophosphate, formed by thioesterification of free fatty acids with ATP and CoA with the aid of acyl-CoA synthetase (EC 6.2.1.3) to inorganic phosphate. This is measured using the reaction with molybdate. The reaction equations are as follows: