Fluoride is an inorganic and monoatomic anion with the chemical formula F-. It occurs naturally in water, air and soil. It has effects such as neurotoxic, genotoxic, oxidative damage and deterioration of cognitive skills on public health. Fluoride can be taken into the body from various sources such as drinking water, food, drugs, industrial and cosmetic products. Commercially available toothpastes and mouthwashes are also sources of fluoride and increase the fluoride concentration in the body. Fluoride concentrations in toothpastes and mouthwash solutions range from 230-1500 mg/L. Insufficiency of swallowing reflex in preschool children causes higher fluoride intake. In the study, the effect of fluoride taken at certain doses on antioxidant enzyme activities in zebrafish liver tissue was investigated. In the study, 1.5, 5 and 100 ppm fluoride solutions were applied to adult zebrafish for 6 weeks, taking into account the allowed doses. After chronic exposure, fish were dissected and liver tissues were taken. Then, SOD, CAT and GPx enzyme activities in these tissues were examined. When the results obtained are compared with the control group, it was found that SOD activity did not show a significant change at 1.5 and 5 ppm, but showed a significant decrease at 100 ppm. When CAT activity is evaluated, while a statistically significant increase was observed in the activity at 1.5, 5 and 100 ppm doses compared to the control group. Also, it was determined that the three doses applied did not show a significant change in the GPx activity. Considering all the results obtained, it has been observed that chronic fluorine application at low doses affects the antioxidant enzyme system by creating oxidative damage in zebrafish.