Akademik Veri Yönetim Sistemi
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.19, sa.10, ss.2151-2158, 2010 (SCI-Expanded, Scopus)
The aim of this study was to evaluate the possible nephroprotective and hepatoprotective effects of grape seed extract (GSE), an antioxidant agent, against doxorubicin (DOX)-induced nephrotoxicity and hepatotoxicity in mice using biochemical and histopathological approaches. Swiss albino mice were randomly divided into six groups, each consisting of six animals: control; DOX (2.5 mg/kg body weight, 6-days cumulative dosage 15 mg/kg b. wt); GSE (50 mg/kg b. wt); GSE (150 mg/kg b. wt); GSE (50 mg/kg b. wt) + DOX; and GSE (150 mg/kg b. wt) + DOX. Serum aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels were determined to assess the liver and kidney functions, respectively. Besides, the liver and kidney samples were taken for the determination of tissue malondialdehyde and glutathione levels, as well as histopathological changes in the tissues. The results indicated that DOX-induced oxidative damage caused a significant decrease in glutathione levels, but a significant increase in malondialdehyde levels of the liver and kidney tissues. Each dose of GSE provided significant protection against DOX-induced toxicity, and the strongest effect was observed at a dose of 150 mg/kg body weight. Histopathological studies showed that DOX caused some structural damages, such as vascular congestion, hepatocyte and tubular epithelial degeneration, hemorrhage, glomerular atrophy and necrosis, hyaline casts in kidney, and fibrosis in the liver and kidney. In vivo results showed that GSE extract is a potent protector against DOX-induced nephrotoxicity and hepatotoxicity.