HPLC Method for Determination of Atenolol in Human Plasma and Application to a Pharmacokinetic Study in Turkey


YILMAZ B., ARSLAN S., AŞCI A.

JOURNAL OF CHROMATOGRAPHIC SCIENCE, cilt.50, sa.10, ss.914-919, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 10
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1093/chromsci/bms090
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHIC SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.914-919
  • Atatürk Üniversitesi Adresli: Evet

Özet

This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The calibration curve was linear within the concentration range of 5-150 ng/mL. Intra-day and inter-day precision values for atenolol in plasma were less than 6.1, and accuracy (relative error) was better than 5.5%. The mean recovery of atenolol was 98.4% for plasma. The limits of detection and quantification of atenolol were 1.5 and 5 ng/mL, respectively. Also, this assay was successfully applied to six patients with hypertension who had been given an oral tablet of 50 mg atenolol.