Akademik Veri Yönetim Sistemi
Turkish Journal of Nature and Science, cilt.10, sa.1, ss.254-258, 2021 (Hakemli Dergi)
Streptomyces is a genus of Gram-positive bacteria that grows in different habitats, and
its shape takes after filamentous fungi. The most effective characteristic of Streptomyces is the
ability to produce secondary metabolites, such as antivirals, antifungals, anti-hypertensives,
antitumorals, and especially antibiotics. In this study, bacteria producing antimicrobial substances
were isolated from soil samples collected from Palandöken Mountain. Test strains were identified
using conventional (morphological, physiological and biochemical tests) and molecular methods
(16S rRNA sequencing). Then, the antagonistic effect of these bacteria against pathogenic
microorganisms was determined by disc diffusion method. As a result of analysis, it was found
that two bacteria (AO1 and AO3) were similar to Streptomyces violaceochromogenes, one (AO2)
to Streptomyces ambofaciens and other (AO4) to Sphingomonas melonis at a rate of 99%.
According to conventional tests, all isolates were catalase positive, three were oxidase negative
(except AO4). In addition, pH, NaCl and temperature values that isolates can growth were
determined. Also, phylogenetic trees of the isolates were performed by the neighbor-joining
method. Finally, the antimicrobial properties of bacteria were investigated. It was determined that
the isolates generally showed high antimicrobial effect against Escherichia coli O157: H7 strain
and the lowest antimicrobial effect was shown against Staphylococcus aureus strain