Bimetallic Nanozyme-Assisted Immunoassay for the Detection of Acetamiprid in Vegetables


Liu B., Zhai R., Abd El-Aty A. M. A., Zhang J., Liu G., Huang X., ...Daha Fazla

ACS APPLIED NANO MATERIALS, cilt.7, sa.18, ss.21833-21841, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 18
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1021/acsanm.4c03806
  • Dergi Adı: ACS APPLIED NANO MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex
  • Sayfa Sayıları: ss.21833-21841
  • Anahtar Kelimeler: Acetamiprid, Bimetallic Au@Pt nanozyme, Indirect competitive immunoassay, Residue, Vegetables
  • Atatürk Üniversitesi Adresli: Evet

Özet

The ability of conventional enzyme-linked immunosorbent assays to satisfy the increasing demand for advanced immunoassays is limited by their reliance on natural enzymes, low sensitivity, and narrow detection range. The development of nanozyme-assisted immunoassays is a viable approach to overcoming these constraints. Here, we report a one-step bimetallic nanozyme-assisted indirect competitive immunoassay to detect the pesticide acetamiprid in vegetables. Litchi-like bimetallic gold and platinum (Au@Pt) nanozymes with high catalytic properties and excellent biocompatibility were synthesized for this assay. The Au@Pt nanozymes were simple and inexpensive to prepare, exhibited stability and adjustable catalytic activity, and were directly conjugated with antibodies as signal probes, without the use of horseradish peroxidase-conjugated Affinipure goat antimouse IgG (IgG-HRP). Acetamiprid was shown to compete with the antigen to bind the Au@Pt probes. Subsequently, Au@Pt probes, which exhibited peroxidase-like activity, were used to catalyze the oxidation of colorless 3,3 ',5,5 '-tetramethylbenzidine, demonstrating the easy detection of acetamiprid through colorimetric signal monitoring. The linear range, sensitivity, relative standard deviation range, and limit of detection of the immunoassay were 1.85-327.19 mu g/L, 25.58 mu g/L, 0.46-10.35%, and 0.78 mu g/L, respectively, satisfying the detection requirements. In conclusion, the method exhibited high sensitivity, reproducibility, and a wide linear range for the detection of acetamiprid, while eliminating the need for IgG and simplifying the experimental procedure. This new indirect competitive immunoassay has great potential value for on-site sensitive detection of acetamiprid in vegetables.