Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Akdemir F. N. E., Guelcin I., Karagoez B., SOSLU R.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.31, ss.162-166, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/14756366.2016.1193735
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.162-166
  • Anahtar Kelimeler: Antioxidant enzymes, ischemia reperfusion, quercetin, skeletal muscle, IN-VITRO ANTIOXIDANT, RADICAL SCAVENGING ACTIVITY, CHRONIC CADMIUM NEPHROTOXICITY, LYOPHILIZED AQUEOUS EXTRACT, ANTIRADICAL ACTIVITIES, POLYPHENOL CONTENTS, N-ACETYLCYSTEINE, LIPID-PEROXIDATION, LIMB ISCHEMIA, CAFFEIC ACID
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, we investigated the potential beneficial effects of quercetin on skeletal muscle ischemia reperfusion injury. Twenty-four Sprague-Dawley type rats were randomly divided into four groups. In the sham group, only gastrocnemius muscle were removed and given no quercetin. In ischemia group, all the femoral artery, vein and collaterals were occluded in the left hindlimb by applying tourniquate under general anaesthesia for three hours but reperfusion was not done. In the Quercetin+Ischemia reperfusion group, quercetin (200mg kg(-1) dose orally) was given during one-week reoperation and later ischemia reperfusion model was done. Finally, gastrocnemius muscle samples were removed to measure biochemical parameters. The biomarkers, MDA levels, SOD, CAT and GPx activities, were evaluated related to skeletal muscle ischemia reperfusion injury. MDA levels reduced and SOD, CAT and GPx activities increased significantly in Quercetin+Ischemia reperfusion group. Results clearly showed that Quercetin have a protective role against oxidative damage induced by ischemia reperfusion in rats.