Evaluation of New Bone Formation Using Autogenous Tooth Bone Graft Combined with Platelet-Rich Fibrin in Calvarial Defects

KIZILDAĞ A., Tasdemir U., ARABACI T., ÖZMEN Ö., Kizildag C. A., Iyilikci B.

JOURNAL OF CRANIOFACIAL SURGERY, cilt.30, sa.6, ss.1662-1666, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1097/scs.0000000000005413
  • Dergi Adı: JOURNAL OF CRANIOFACIAL SURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1662-1666
  • Anahtar Kelimeler: Bone formation, calvarial defects, platelet-rich fibrin, tooth graft, CALCIUM-PHOSPHATE CERAMICS, DENTIN MATRIX, INTRABONY DEFECTS, GROWTH-FACTORS, AUGMENTATION, EXPRESSION, ALLOGRAFT, IMPLANTS, SOCKETS, RABBITS
  • Atatürk Üniversitesi Adresli: Evet

Özet

The purpose of the present study was to evaluate the contributions of autogenous tooth bone graft (ATBG) combined with platelet-rich fibrin (PRF) on new bone formation and bone morphogenetic protein (BMP)-2 in rabbit calvarial defects. Twelve male New Zealand rabbits were used in this study. Three circular bone defects were prepared in each rabbit with a drill. These defects were divided into 3 groups: control, treated with ATBG, and treated with ATBG+PRF. The animals were sacrificed at 28 days. Samples were evaluated by histomorphometric analyses and total augmented area, new bone area and bone density were calculated. In addition, expression of BMP-2 was determined by immunohistochemical staining. The total augmented area, new bone area and bone density were significantly greater in the ATBG group than in the control group (P<0.05). Also, these values were significantly higher in the ATBG+PRF group than the ATBG group (P<0.05). Test groups demonstrated significantly increased BMP-2 levels compared with the control group (P<0.05). The present study suggested that ATBG combined with PRF significantly increased the new bone formation and enhanced bone healing in cranial defects.