A novel bacteriocin from Lacticaseibacillus rhamnosus with potent anti-Vibrio activity: Purification, characterization and application in shrimp preservation
Food Control, cilt.193, 2027 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 193
- Basım Tarihi: 2027
- Doi Numarası: 10.1016/j.foodcont.2026.112637
- Dergi Adı: Food Control
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Index Islamicus, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO)
- Anahtar Kelimeler: Antibacterial mechanism, Bacteriocin, Lacticaseibacillus rhamnosus, Shrimp, Vibrio parahaemolyticus
- Atatürk Üniversitesi Adresli: Evet
Özet
The increasing prevalence of antibiotic-resistant pathogens, particularly in seafood, necessitates the discovery of novel biopreservatives. This study reports the isolation of a Lacticaseibacillus rhamnosus SLG21 strain from fermented fish sauce, which exhibits exceptional tolerance to high selenite concentrations (5 mM) and potent antagonistic activity against foodborne pathogens. A novel bacteriocin, designated SLG21, was purified and identified as a 16-residue cationic peptide (KYGNGGLSRIFSALLK, 1554.15 Da). Bacteriocin SLG21 demonstrated broad-spectrum bactericidal activity, with minimum inhibitory concentrations (MICs) of 4–16 μg/mL against Gram-positive pathogens and, crucially, 16–128 μg/mL against Gram-negative pathogens. Mechanistic studies revealed that the bacteriocin SLG21 overcomes the Gram-negative outer membrane barrier through high-affinity binding to LPS, leading to outer membrane permeabilization and subsequent inner membrane depolarization. Toxicology assessments confirmed its biosafety at preservative-relevant concentrations. In a shrimp model inoculated with Vibrio parahaemolyticus, bacteriocin SLG21 (1× MIC) rapidly inhibited bacterial growth and delayed chemical spoilage (as measured by total volatile basic nitrogen). Although its recoverable activity decreased to 28.5% by day 8, the initial bactericidal action may suggest potential application in Pacific white shrimp against V.parahaemolyticus.