Akademik Veri Yönetim Sistemi
Anatolian Journal of Biology, cilt.1, sa.1, ss.38-44, 2021 (Hakemli Dergi)
The latex of the Euphorbia plant was used in this study to find an effective and natural new insecticide in
combating Dentroctonus micans (Kug.), Which causes great damage to Picea orientalis forests. For this purpose;
Euphorbia plant was collected from Erzurum and species identification was made. According to the species
identification, the spurge plant was determined to be Euphorbia virgata, and then its latex was obtained. In this
study; Different concentrations of latex (0, 25, 50 and 75%) were applied to the experimental groups prepared to
have fifty D. micans larvae in each group, and the experiment was repeated twice. As a result of the application,
the LD50 (25%, 50% and 75%) values, respectively; 0.01µl/ml, 0.196 µl/ml, 0.294 µl/ml. LD90 values were found
as 0.18 µl/ml, 0.354 µl/ml, and 0.531 µl/ml. Although E. virgata latex has a toxic effect on the larvae at any
concentration, the mortality rate increased as the concentration amount increased. GC-MS analysis was performed
to determine the latex content of E. virgata. According to GC-MS analysis, in latex content; there are terpenes,
flavonoids, alkaloids, amines, fatty acids, steroids and various alcohols. These bioactive substances in E. virgata
latex showed toxic effects on larvae. As a result of the study, it was found that E. virgata latex can be used as a
natural and effective insecticide in the fight against D. micans..