Cytotoxic naphthoquinones fromArnebia densiflora(Nordm.) Ledeb and determining new apoptosis inducers

Baran M. Y., GÜVENALP Z., SARAÇOĞLU İ., KAZAZ C., SALİH B., DEMİREZER L. Ö., ...Daha Fazla

NATURAL PRODUCT RESEARCH, cilt.34, sa.12, ss.1669-1677, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/14786419.2018.1525714
  • Dergi Adı: NATURAL PRODUCT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1669-1677
  • Anahtar Kelimeler: Alkannin, cytotoxicity, caspase, apoptosis, Arnebia densiflora, Boraginaceae, ARNEBIA, SHIKONIN, CALLUS
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, phytochemical composition ofArnebia densiflora(AD) was determined and cytotoxic effects of then-hexane extract and compounds isolated from this species on various cell lines were investigated. By means of serial chromatographic studies, 6 naphthoquinone derivatives were yielded, which are isovalerylalkannin,alpha-methyl-n-butyl alkannin, acetylalkannin,beta-acetoxy isovalerylalkannin, alkannin and a new compound: 4-hydroxy 4-methyl valeryl alkannin. Structures of the isolated compounds were elucidated using UV, IR, 1D-2D NMR, MS and CD methods. Cytotoxic effects of the extract and isolated alkannins were investigated on L929, HeLa, HEp-2 cells. AD and the isolated compounds demonstrated moderate to strong cytotoxic effects (IC(50)range: 4.92-172.35 mu g/ml). The results of DNA fragmentation and caspase-3 activity studies on HeLa cells exhibited that AD and the naphthoquinones isolated from it caused cytotoxicity through induction of apoptosis.