Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.45, sa.4, ss.454-464, 2021 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 45 Sayı: 4
- Basım Tarihi: 2021
- Doi Numarası: 10.3906/tar-1910-61
- Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Compendex, Environment Index, Geobase, INSPEC, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.454-464
- Anahtar Kelimeler: Culture media, dicamba, picloram, plant regeneration, 2.4-D, SOMATIC EMBRYOGENESIS, SHOOT FORMATION, AUXIN, CULTIVARS, GENOTYPES, EXPLANTS, IMMATURE
- Atatürk Üniversitesi Adresli: Evet
Özet
To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/l, 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/l.2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.