Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)

KODAZ S., HALİLOĞLU K., Pour A. H., AYDIN M.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.45, sa.4, ss.454-464, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3906/tar-1910-61
  • Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Compendex, Environment Index, Geobase, INSPEC, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.454-464
  • Anahtar Kelimeler: Culture media, dicamba, picloram, plant regeneration, 2.4-D, SOMATIC EMBRYOGENESIS, SHOOT FORMATION, AUXIN, CULTIVARS, GENOTYPES, EXPLANTS, IMMATURE
  • Atatürk Üniversitesi Adresli: Evet

Özet

To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/l, 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/l.2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.