Genetic relationships among wild pomegranate (Punica granatum) genotypes from Coruh Valley in Turkey


ERCİŞLİ S., GADZE J., AĞAR G., YILDIRIM N., HIZARCI Y.

GENETICS AND MOLECULAR RESEARCH, cilt.10, sa.1, ss.459-464, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.4238/vol10-1gmr1155
  • Dergi Adı: GENETICS AND MOLECULAR RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.459-464
  • Anahtar Kelimeler: Wild pomegranate, Punica granatum, RAPD, L., RAPD, DIVERSITY, CULTIVARS, REGION
  • Atatürk Üniversitesi Adresli: Evet

Özet

The pomegranate has been used traditionally in Coruh Valley in Turkey for a long time; fruits are harvested from wild, semi-domesticated and cultivated trees. In the valley, it occurs in general along with olive trees. We sampled 23 wild-grown pomegranate genotypes sampled from different parts of Coruh Valley and compared them using RAPD primers to determine genetic variability. Eighty-six RAPD primers were used for molecular characterizations, among which 12 gave reliable polymorphic patterns. These primers generated 145 RAPD bands of which 91% were polymorphic. The highest polymorphism ratio was observed with primers OPY06, OPY13, OPBA03, OPBB03, OPBB07, and OPBB09 (100%), while the lowest was with OPBB09 and OPBB10 (75%). The band size was between 250 and 2400 bp. There were five main clusters in the dendrogram; the highest genetic similarity was 0.24 and the lowest was 0.08.