Evaluation of the Antioxidant and Antiradical Properties of Some Phyto and Mammalian Lignans

Polat Kose L., GÜLÇİN İ.

MOLECULES, cilt.26, sa.23, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 23
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3390/molecules26237099
  • Dergi Adı: MOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: phenolic compounds, lignan, antioxidant activity, antiradical activity, reducing power, IN-VITRO ANTIOXIDANT, RADICAL SCAVENGING ACTIVITIES, LYOPHILIZED AQUEOUS EXTRACT, POLYPHENOL CONTENTS, CARBONIC-ANHYDRASE, ANTICHOLINERGIC PROPERTIES, ACETYLCHOLINESTERASE, METABOLISM, SECOISOLARICIRESINOL, DERIVATIVES
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, the antioxidant and antiradical properties of some phyto lignans (nordihydroguaiaretic acid, secoisolariciresinol, secoisolariciresinol diglycoside, and alpha-(-)-conidendrin) and mammalian lignans (enterodiol and enterolactone) were examined by different antioxidant assays. For this purpose, radical scavenging activities of phyto and mammalian lignans were realized by 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS(center dot+)) scavenging assay and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging assay. Additionally, the reducing ability of phyto and mammalian lignans were evaluated by cupric ions (Cu2+) reducing (CUPRAC) ability, and ferric ions (Fe3+) and [Fe3+-(TPTZ)2](3+) complex reducing (FRAP) abilities. Also, half maximal inhibitory concentration (IC50) values were determined and reported for DPPH center dot and ABTS(center dot+) scavenging influences of all of the lignan molecules. The absorbances of the lignans were found in the range of 0.150-2.320 for Fe3+ reducing, in the range of 0.040-2.090 for Cu2+ reducing, and in the range of 0.360-1.810 for the FRAP assay. On the other hand, the IC50 values of phyto and mammalian lignans were determined in the ranges of 6.601-932.167 mu g/mL for DPPH center dot scavenging and 13.007-27.829 mu g/mL for ABTS(center dot+) scavenging. In all of the used bioanalytical methods, phyto lignans, as secondary metabolites in plants, demonstrated considerably higher antioxidant activity compared to that of mammalian lignans. In addition, it was observed that enterodiol and enterolactone exhibited relatively weaker antioxidant activities when compared to phyto lignans or standard antioxidants, including butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), Trolox, and alpha-tocopherol.