Some indazoles as alternative inhibitors for potato polyphenol oxidase

Ozturk C., Bayrak S., Demir Y., Aksoy M., Alim Z., Özdemir H., ...Daha Fazla

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.69, sa.5, ss.2249-2256, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/bab.2283
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.2249-2256
  • Anahtar Kelimeler: affinity chromatography, enzyme purification, indazoles, inhibition, polyphenol oxidase, IN-SILICO EVALUATION, CARBONIC-ANHYDRASE, POTENTIAL ACETYLCHOLINESTERASE, PARTIAL-PURIFICATION, VITRO, TYROSINASE, L., KINETICS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Fresh-cut vegetables and fruits have gained attention among consumers because of their fresh appearance, lack of pollution, nutrition, and convenience. However, in fresh-cut foods, enzymatic browning is the main problem. Polyphenol oxidase (PPO) is a vital enzyme involved in the process of enzymatic browning. In this study, PPO was purified from potato using Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity chromatography and the effect of some indazoles on the enzyme was determined. The enzyme was purified with a specific activity of 52,857.14 EU/mg protein and 21.26-purification fold. Indazoles exhibited inhibitor properties for PPO with IC50 values in the range of 0.11-1.12 mM and K-i values in the range of 0.15 +/- 0.04-3.55 +/- 0.88 mM. Among these compounds, 7-chloro-1H-indazole was shown as the most potent PPO inhibitor (K-i: 0.15 +/- 0.04 mM). Determination of the enzyme's inhibition kinetics will simplify the testing of candidate PPO inhibitors.