Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson's disease

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Ferah Okkay I., Okkay U., Cicek B., Yilmaz A., Yesilyurt F., Mendil A. S., ...Daha Fazla

MOLECULAR BIOLOGY REPORTS, cilt.48, sa.12, ss.7711-7717, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11033-021-06779-y
  • Dergi Adı: MOLECULAR BIOLOGY REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.7711-7717
  • Anahtar Kelimeler: 6-OHDA, Bromelain, Neuroprotection, Parkinson's disease, SH-SY5Y cell, CELL-DEATH, SH-SY5Y CELLS, TOXICITY, APOPTOSIS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Background This study was designed to investigate the neuroprotective effects of bromelain, which is known to have anti-oxidant and anti-inflammatory properties, against the neurotoxicity (induced by 6-OHDA) in SH-SY5Y cells. Methods and results To establish Parkinson's Disease (PD) model in cell culture conditions, SH-SY5Y cells were exposed to 200 mu M 6-OHDA for 1 day. Prior to 6-OHDA treatment, SH-SY5Y cells had been pre-treated with bromelain (25 mu g/mL, 50 mu g/mL, 75 mu g/mL and 100 mu g/mL). After 1 day, cell viability was determined with the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and lactate dehydrogenase (LDH) assays. Oxidative stress was assessed with total antioxidant capacity (TAC), total oxidant status (TOS), glutathione reductase (GR) and malondialdehyde (MDA) analyses. The effect of the bromelain in SH-SY5Ycells was also examined by 4 ',6-diamidino-2-phenylindole (DAPI) staining. We found that 6-OHDA increased LDH leakage, and cellular apoptosis in SH-SY5Y cells. 6-OHDA aggravated oxidative stress by increasing TOS, MDA and GR and eventually promoted apoptosis in SH-SY5Y cells, while pretreatment with bromelain attenuated these toxic effects of 6-OHDA. Conclusions These findings indicated that bromelain, with its neuroprotective features can be useful for neuroprotection in PD.