Akademik Veri Yönetim Sistemi
ARCHIVES OF MICROBIOLOGY, cilt.208, sa.1, 2025 (SCI-Expanded, Scopus)
Q fever is a globally prevalent zoonotic disease, with the exception of New Zealand and French Polynesia. This study aimed to investigate the presence of gene regions encoding Com1, porin P1, CB-MIP, and heat shock proteins in C. burnetii-positive tissue samples collected from the Veterinary Control Institutes in Erzurum, Samsun, Pendik, and Konya using PCR, and to produce the Com1 protein recombinantly from a selected local isolate. PCR analysis revealed the presence of heat shock protein genes in 82.3% of the positive samples, Porin P1 in 70.5%, CB-MIP in 94.1%, and Com1 in 88.2%. The com1 gene from one field isolate was successfully cloned using the Champion (TM) pET Directional TOPO (R) Expression Kit, and the recombinant protein was expressed. The immunological activity of the resulting proteins, generated using three different primer combinations, was assessed via ELISA. Among the tested proteins, Protein Com1A demonstrated superior immunoreactivity compared to Proteins Com1B and Com1C. Considering the requirement for Biosafety Level 3 (BSL-3) laboratories for the cultivation of the bacterium, the successful recombinant expression of the immunodominant Com1 antigen offers a valuable tool for the development of diagnostic kits or vaccine candidates.