Conducting Polymer-Reduced Graphene Oxide Sponge Electrode for Electrochemical Detection Based on DNA Hybridization

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Dağcı Kıranşan K., Topçu E.

ACS APPLIED NANO MATERIALS, cilt.3, sa.6, ss.5449-5462, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1021/acsanm.0c00782
  • Dergi Adı: ACS APPLIED NANO MATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex
  • Sayfa Sayıları: ss.5449-5462
  • Anahtar Kelimeler: Sponge electrode, reduced graphene oxide, conductive polymer, DNA detection, methylene blue, INITIATED ENZYMATIC POLYMERIZATION, LABEL-FREE, 3-DIMENSIONAL GRAPHENE, HIGH-PERFORMANCE, PAPER ELECTRODE, COMPOSITE PAPER, FABRICATION, ADSORPTION, FILMS, BINDING
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, polymer-supported graphene-based material was designed for electrochemical determination based on the hybridization of DNA. First, dispersions containing graphene oxide (GO) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) polymer in different compositions were transformed into a hydrogel form through the hydrothermal treatment, then 3D and flexible PEDOT:PSS/reduced graphene oxide (RGO) composite sponge material was prepared by using a freeze-drying process. Structural and morphological characterizations showed that PEDOT:PSS polymer served as a skeleton by covering the graphene layers like a mesh and provided an increment in both mechanical strength and electrochemical performance by relatively reducing pore size in 3D material. PEDOT:PSS/RGO composite sponge has high conductivity (158 S cm(-1)) for utilization as an electrode and durability to lift 700 times its weight. For electrochemical determination based on the hybridization of DNA on PEDOT:PSS/RGO composite sponge material, single-stranded DNA (ssDNA) and methylene blue (MB) marked ssDNA probes were immobilized on PEDOT:PSS/RGO composite sponge material at optimum conditions. This sensor achieved a detection limit down to 17 fM with the linear range of 50 fM to 2 mu M for the determination of hybridized DNA. By possessing flexible, free-standing, and durable features, PEDOT:PSS/RGO composite sponge appeared to be a promising electrode material for detection studies.