Purification and characterization of the carbonic anhydrase enzyme from horse mackerel (Trachurus trachurus) muscle and the impact of some metal ions and pesticides on enzyme activity

Caglayan C., Taslimi P., Turk C., Kandemir F. M., Demir Y., Gülçin İ.

Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, cilt.226, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 226
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.cbpc.2019.108605
  • Dergi Adı: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Trachurus trachurus, Horse mackerel, Enzyme purification, Enzyme characterization, Carbonic anhydrase, Heavy metal, Pesticides, HEAVY-METALS, OXIDATIVE STRESS, 1ST SYNTHESIS, LIVER, FISH, INHIBITION, ACETYLCHOLINESTERASE, DERIVATIVES, PRODUCTS, COPPER
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this paper, the total carbonic anhydrase (CA) enzyme was purified from horse mackerel (Trachurus trachurus) muscle with a specific activity of 23,063.93 EU/mg, purification fold of 551.08, total activity of 1522.22 EU/mL and a yield of 18.50% using sulfanilamide affinity column chromatography. For obtaining the subunit molecular mass and enzyme purity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for this part was performed and a single band was clearly recorded. The molecular mass of this enzyme was found approximately 35 kDa. The optimum temperature and pH values were obtained from Arrhenius plot. In addition, the inhibitory effects of different heavy metal ions (Fe2+, Cu2+, Co2+, Pb2+ Hg2+ and As3+) and some pesticides (thiram, clofentezine, propineb, deltamethrin, azoxystrobin and thiophanate) on horse mackerel (Trachurus trachurus) muscle tissue CA enzyme activities were investigated by utilizing esterase assay activity. The used metal ions and pesticides had IC50 values in the range of 0.21-13.84 mM and 3.78-70.58 mM, respectively.