Plant regeneration in wheat mature embryo culture

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Aydın M., Tosun M., Haliloğlu K.

AFRICAN JOURNAL OF BIOTECHNOLOGY, cilt.10, sa.70, ss.15749-15755, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 70
  • Basım Tarihi: 2011
  • Doi Numarası: 10.5897/ajb11.1495
  • Dergi Adı: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.15749-15755
  • Anahtar Kelimeler: Callus formation, explant, genotype, plant growth regulators, TRITICUM-AESTIVUM, CALLUS INDUCTION, TISSUE-CULTURE, IMMATURE EMBRYOS, GENOTYPE, INITIATION, CULTIVARS, EXPLANTS, GROWTH, BARLEY
  • Atatürk Üniversitesi Adresli: Evet

Özet

Success in genetic engineering of cereals depends on the callus formation and efficient plant regeneration system. Callus formation and plant regeneration of wheat mature embryos were assessed by using 12 different methods with 4 genotypes. Genotype significantly affected the formation of callus, embryogenic callus and plant regeneration. Only two methods (methods # 2 and 9) produced plant regeneration. The highest plant regeneration for all genotypes with endosperm that supported mature embryos was observed in method # 9 which contain MS medium supplemented with 12 mg/l dicamba + 0.5 mg/l IAA.