Influence of Cobalt and Zinc Exposure on mRNA Expression Profiles of Metallothionein and Cytocrome P450 in Rainbow Trout

CEYHUN S. B., Aksakal E., Ekinci D., ERDOĞAN O., Beydemir S.

BIOLOGICAL TRACE ELEMENT RESEARCH, cilt.144, ss.781-789, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 144
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s12011-011-9068-z
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.781-789
  • Anahtar Kelimeler: Gene expression, Metallothionein, Cytocrome P450 1A, Heavy metal, CYTOCHROME-P450 1A, GENE-EXPRESSION, FRESH-WATER, OXIDATIVE STRESS, HEAVY-METALS, CADMIUM, PROTEIN, TOXICITY, ACCUMULATION, RESPONSES
  • Atatürk Üniversitesi Adresli: Evet

Özet

The present research aims to evaluate the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on metallothioneins and cytocrome P450. Mature rainbow trouts were exposed to 10 mg/L CoCl2.6H(2)O and 1 mg/L ZnSO4.7H(2)O. After 6, 12, 24, and 48 h of treatment, expressions of muscle MT-A, MT-B, and CYP P4501A1 mRNAs were measured by means of quantitative real-time polymerase chain reaction. During the exposure experiments, no mortalities occurred. We observed that expression levels of all genes increased with exposure time. Since the organism has not learned how to completely dispose of heavy metals and tends to bioaccumulate them, our results indicate that cobalt and zinc exposure may result in accumulation of the non-eliminated metals which may lead to fish death.