A dummy molecularly imprinted solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for selective determination of four pyridine carboxylic acid herbicides in milk

Tan, Sijia; Yu, Hailong; He, Yahui; Wang, Miao; Liu, Guiqiao; Hong, Sihui; Yan, Feiyan; Wang, Yanli; Wang, MengQiang; Li, Tengfei; Wang, Jing; Abd El-Aty, ABDELATY; Haclmuftuoglu, Ahmet; She, Yongxin

doi:10.1016/j.jchromb.2019.01.008

A dummy molecularly imprinted solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for selective determination of four pyridine carboxylic acid herbicides in milk

Tan S., Yu H., He Y., Wang M., Liu G., Hong S., ...Daha Fazla

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, cilt.1108, ss.65-72, 2019 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 1108
Basım Tarihi: 2019
Doi Numarası: 10.1016/j.jchromb.2019.01.008
Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.65-72
Anahtar Kelimeler: Molecularly imprinted polymers, Pyridine carboxylic acid herbicides, Solid-phase extraction, Milk, Residue determination, POLYMERS, SAMPLES, RESIDUES, WATER, FOOD
Atatürk Üniversitesi Adresli: Evet

Özet

We aim to develop a solid-phase extraction (SPE) based on a dummy molecularly imprinted polymer (MIP) and liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS) for selective determination of four pyridine carboxylic acid herbicides (aminopyralid, picloram, fluroxypyr, and clopyralid) in milk samples. Using picloram as the dummy template, MIP nanocomposites with highly selective recognition and adsorption for the target molecule and its structural analogs were successfully synthesized by precipitation polymerization. Adsorption isotherms and kinetics were used to determine the adsorption performance and specific recognition mechanism of both MIPs and non-molecularly imprinted polymers (NIP). The Scatchard plot analysis revealed that two different binding sites were formed in the MIP with maximum binding capacity (Q(max)) of 1171.8 mu g.g(-1) and 3022.5 mu g.g -1 , respectively. Recovery at three spiking levels of 10, 20, and 50 mu g.L-I ranged between 75.3 and 89.8% with relative standard deviations (RSDs) < 14.3%.The limit of detection (LOD) was estimated to be 0.124 mu g.L-1. Finally, the feasibility of the proposed methodology was successfully applied to quantify aminopyralid and another three pyridine carboxylic acid herbicides in milk.