Comparative Analysis of Far East Sikhotinsky Rhododendron (Rh. sichotense) and East Siberian Rhododendron (Rh. adamsii) Using Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Razgonova M., Zakharenko A., ERCİŞLİ S., Grudev V., Golokhvast K.

MOLECULES, cilt.25, sa.17, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 17
  • Basım Tarihi: 2020
  • Doi Numarası: 10.3390/molecules25173774
  • Dergi Adı: MOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Anahtar Kelimeler: Rhododendron sichotense, Rhododendron adamsii, supercritical fluid extraction, HPLC-MS, MS, phenolic compounds, LEDUM-PALUSTRE ERICACEAE, ESSENTIAL OIL, EXTRACTION, LEAVES, FLAVONOIDS, L., CHROMATOGRAPHY, ACIDS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Rhododendron sichotensePojark. andRhododendron adamsiiRheder have been actively used in ethnomedicine in Mongolia, China and Buryatia (Russia) for centuries, as an antioxidant, immunomodulating, anti-inflammatory, vitality-restoring agent. These plants contain various phenolic compounds and fatty acids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) extraction has been shown to be the method of choice for the recovery of these naturally occurring compounds. Operative parameters and working conditions have been optimized by experimenting with different pressures (300-400 bar), temperatures (50-60 degrees C) and CO(2)flow rates (50 mL/min) with 1% ethanol as co-solvent. The extraction time varied from 60 to 70 min. A HPLC-UV-VIS-ESI-MS/MS technique was applied to detect target analytes. A total of 48 different biologically active components have been identified in theRh. adamsiiSC-CO(2)extracts. A total of 31 different biologically active components have been identified in theRh. sichotenseSC-CO(2)extracts.