Akademik Veri Yönetim Sistemi
International Journal of Biological Macromolecules, cilt.309, sa.3, 2025 (SCI-Expanded)
This study investigates the potential of 5 % pullulan (PUL)-based edible coating to extend the shelf life and maintain the postharvest quality of ‘Furongli’ plums stored at 25 ± 2 °C and 75–80 % relative humidity for 12 days. PUL-coated plums exhibited 31 and 72.7 % lower weight loss rate and disease incidence compared to uncoated plums after 12 days of storage, respectively. The coating significantly reduced oxidative stress markers, including MDA (22 %), H2O2 (25.3 %), and O2•− (25.1 %), while improving membrane integrity by reducing electrolyte leakage by 18 %. Additionally, the coating delayed carotenoid degradation by 81.3 % and preserved the fruit's visual appearance by reducing hue and chroma changes by 31.7 % and 30.8 %, respectively. Firmness was better maintained, along with reduced respiration and ethylene production in coated plums. Biochemical analysis revealed that PUL coatings enhanced antioxidant defense by increasing both the activities and gene expression of antioxidant enzymes (SOD, POD, and CAT) and maintained higher levels of ascorbic acid, total phenolics, flavonoids, and overall antioxidant activity. The coating preserved cell wall components like hemicellulose, cellulose, and protopectin while limiting increases in water-soluble pectin, thus delaying fruit softening. Additionally, PUL coating reduced activities and gene expression of pectin-degrading enzymes (PME and PG). Quality parameters, including TSS, TTA, and TSS-acid ratios, remained stable in coated fruits, ensuring better flavor and marketability. Overall, the study highlights the potential of PUL-based coatings for large-scale postharvest application, offering a sustainable and effective solution to extend fruit shelf life and reduce storage losses.