Production of bacterial cellulose by Acetobacter okinawensis AC2 and its application as a bioadsorbent for azo dye removal


ÖMEROĞLU M. A., Keles N., TAŞKIN M.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, 2026 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1002/bbb.70152
  • Dergi Adı: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex, Greenfile, INSPEC
  • Atatürk Üniversitesi Adresli: Evet

Özet

Bacterial cellulose (BC) is a naturally occurring polysaccharide that has attracted considerable interest in various fields, including biological and biomedical applications, due to its biodegradability, biocompatibility, high degree of crystallinity, and outstanding physicochemical characteristics. It is widely used in several industries such as food, pharmaceuticals, and agriculture. In this study, a novel BC-producing bacterial strain, AC2, was isolated from naturally fermented apple vinegar and identified as Acetobacter okinawensis based on a 16S rRNA sequence analysis. Glucose and yeast extracts were determined to be optimal carbon and nitrogen sources, respectively. The highest BC production (5.1 g L-1) was achieved at 28 degrees C, with an initial pH of 6, an inoculum concentration of 7%, an ethanol concentration of 2%, and an incubation time of 10 days under static culture conditions. The presence of functional groups of BC was confirmed by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analysis revealed a nanofibrous network and an intense structure. The potential of BC as a bioadsorbent for azo dye removal was investigated, showing maximum removal of 73% for crystal violet and 49% for Congo red. This is the first report of BC production by A. okinawensis and its application as a bioadsorbent.