BIORESOURCE TECHNOLOGY, cilt.85, sa.2, ss.125-129, 2002 (SCI-Expanded)
Ram horns obtained from the slaughterhouse of Erzurum, Turkey were hydrolyzed by treating with acid (6N-HCl) and ram horn hydrolysate (RHH) was obtained. The hydrolysate was used as substrate to grow Bacillus cereus NRRL B-3711, Bacillus subtilis NRRL NRS-744 and Escherichia coli in batch system at 30 degreesC air 1.5 v/v/min; stirring 150 rpm. Both RHH and biomass samples were analyzed. The bacterial cells produced in this hydrolysate were found to be rich in total protein (66%, 68% and 71% for E. coli, B. cereus and R subtilis, respectively). The chemical oxygen demand and biological oxygen demand were reduced significantly by the growth of bacteria. The protein produced contained all-essential amino acids for ruminant feed. (C) 2002 Elsevier Science Ltd. All rights reserved.