Süleyman Demirel Üniversitesi Ziraat fakültesi Dergisi, cilt.17, sa.2, ss.139-147, 2022 (Hakemli Dergi)
Abstract: This study was carried out in vitro conditions to determine the bacteria
biological control agents of against different fungal agents causing postharvest
losses in lemon fruit. For this purpose, isolations were made from diseased lemon
fruits, as a result of isolations three different fungal species were isolated,
pathogenicity tests were carried out and according to the results of molecular
diagnosis, these isolates were found to be Alternaria alternata (ET 119),
Colletotrichum gleosporoides (ET 120) and Penicillium chrysogenum (ET 121).
Percent inhibition rate of thirty-six antagonist bacterial isolates belonging to eight
different genera against pathogenic fungus isolates was determined by dual
culture test according to a randomized plot experiment design with 3 replications.
According to the results obtained, the highest effect against the three pathogen
isolates was detected in TV 53D (67.46%: Brevibacillus choshinensis), this isolate
was followed by TV 16F (66.27%: Bacillus subtilis) and FDG 37 (62.30%:
Pseudomonas fluorescens) isolates, respectively. More detailed studies should be
carried out in the future under controlled conditions with the most effective
antagonist bacterial isolates.
Keywords: Antifungal activity, Bacillus subtilis, antagonist bacteria, biological
control, Pseudomonas fluorescens