Investigation on effects of walnut essential oil against glutamate toxicity on cortex neuron and LN405 cancer cell lines, diabetes, and some microorganisms

Yesilyurt F., Yuca H., Karakaya S., Tekman E., Demirci B., Taghızadehghalehjoughı A., ...Daha Fazla

JOURNAL OF ESSENTIAL OIL RESEARCH, cilt.35, sa.4, ss.372-381, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 4
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/10412905.2023.2234372
  • Dergi Adı: JOURNAL OF ESSENTIAL OIL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.372-381
  • Anahtar Kelimeler: Walnut, juglans regia, LN405, glutamate, antidiabetic, antimicrobial, >, JUGLANS-REGIA L., VOLATILE OIL, ANTIOXIDANT, LEAVES, NIGRA
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, leaf essential oil effects on glutamate toxicity model formed in cortex neurons and LN405 cell cultures were investigated. Antidiabetic activity was evaluated by & alpha;-amylase and & alpha;-glucosidase inhibitions. MIC was used for antimicrobial activity. Seven groups were examined with MTT. Glutamate 10-5 mM in cortex showed 62% viability whereas oil viability did not increase in a dose-dependent manner and the highest viability rate was observed. There are four types of glandular trichomes in leaf anatomy of walnut. The oil exhibited half as much & alpha;-glucosidase inhibitory activity with an IC50 value of 8105 & mu;g/mL, compared to positive control acarbose (IC50 = 4762 & mu;g/mL). MIC of oil was determined to be 625 & mu;g/mL against E. coli, C. albicans, and C. parapsilosis, whereas S. aureus exhibited a MIC of 1250 & mu;g/mL. Major compounds of oil were found as & beta;-pinene (17.6%), & alpha;-pinene (11.3%), & beta;-eudesmol (8.6%), and caryophyllene oxide (6.2%).