The anti-inflammatory, anti-oxidant and protective effects of a probiotic mixture on organ toxicity in a rat model

Karameşe M., Aydın H., Gelen V., Şengül E., Aksak Karameşe S.

FUTURE MICROBIOLOGY, cilt.15, sa.6, ss.401-412, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.2217/fmb-2020-0005
  • Dergi Adı: FUTURE MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.401-412
  • Anahtar Kelimeler: inflammation, liver and spleen damage, NF-kappa B, probiotics, CARBON-TETRACHLORIDE, OXIDATIVE STRESS, LIVER-INJURY, INDUCED HEPATOTOXICITY, INFLAMMATORY RESPONSE, HEPATIC DAMAGE, IN-VITRO, LACTOBACILLUS, BACTERIA, ALCOHOL
  • Atatürk Üniversitesi Adresli: Evet

Özet

Aim: The objective of this study was to evaluate the possible protective effects of probiotic bacteria, especially Bifidobacterium and Lactobacillus strains, on 4,4 ' -dichlorodiphenyltrichloroethane (DDT)-induced toxicity. For this reason, we evaluated the relationship between probiotics and toxicity by checking immunological and immunohistochemical parameters. Materials & methods: Probiotic pretreatment was applied to 36 Wistar albino rats for 12 consecutive days. Serum aspartate aminotransferase and alanine aminotransferase levels were detected. CD3 and NF-kappa B staining methods were then performed by immunohistochemistry. Finally, pro- and anti-inflammatory cytokines were measured by ELISA. Results: DDT caused a serious increase/decrease in some cytokine parameters. The effective dose was 1 x 10(11) colony-forming unit probiotic treatment. CD3 and NF-kappa B positivity were intense in DDT group whereas the intensity was reduced in probiotic treatment groups. Discussion: The probiotic mixture has a potential to prevent inflammatory and oxidative stress related organ injuries. Further studies should be performed to explain the possible mechanisms.