Akademik Veri Yönetim Sistemi
3. ULUSLARARASI HARRAN SAĞLIK BİLİMLERİ KONGRESİ, 18 Eylül 2021
Objective: Despite the fact that the
negative effects of sulphite on learning and memory have been reported in the
literature, the information on molecular mechanisms is insufficient. In our
study, the effects of different doses of sulfite, which is known to have neurotoxic
effects, on learning and memory were evaluated by behavioral experiments. The
role of ACh in the effects of sulfite on learning and memory, and its possible
mediating mechanisms were investigated.
Method: 3 months old male albino Wistar
rats were divided into three groups. Distilled water was given to control
group, sodium metabisulphite at dose of 100 mg/kg and 260 mg/kg was given to
the sulphite groups for 35 days by gavage. Behavioral changes were evaluated
using the Morris Water Maze, Open Field and New Object Recognition test. After behavioral
experiments were taken, hippocampi were removed under anesthetized. ACh levels,
AChE and ChAT enzyme activities were measured in hippocampus tissues.
Results: It has been observed that the
total distance and average velocity performed in the Open Field test and Morris
Water Maze prop trial were increased, the discrimination index in the New
Object Recognition test decreased in the sulphite applied groups. The amount of
plasma S-sulfonate and ACh in the sulphite applied groups were found to be
increased. While AChE activity decreased significantly in the sulphite applied
groups, ChAT activity increased significantly.
Conclusion: In
conclusion, it has been shown that sulphite intake in adults impairs learning
and spatial memory, and this impairment may be mediated by the cholinergic
signal pathway.