Antioxidant property investigation of a novel plant species discovered from Türkiye: Rheum telianum

Aslan K., Tel A. Z., Yilmaz M. A., Gülçin I.

7. Uluslararası Doğa ve Uygulamalı Bilimlerdeki Gelişmeler Konferansı’na (ICANAS 2024), Antalya, Türkiye, 17 - 20 Nisan 2024, ss.1

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1
  • Atatürk Üniversitesi Adresli: Evet

Özet

Rhubarb, formally known as Rheum spp, is a genus of plant Polygonaceae and is well-known for its rich antioxidant source [1]. The present study, reveals in vitro antioxidant properties and the phytochemical content of a novel Rheum species (Rheum telianum) isolated from Southeastern Anatolia. To perform the study, dried leaves and seeds of the plants were ground and extracted with ethanol to obtain plant secondary metabolites. Then, dried extracts were subjected to in vitro 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and Cupric reducing antioxidant capacity (CUPRAC), Fe3+, and the ferric reducing antioxidant power (FRAP)-reducing assays and all results were compared with the commercially available synthetic and natural antioxidants[2-4]. In addition to the antioxidant capacity assays, quantitative phenolic, flavonoid, and secondary metabolite determination were performed through both colorimetric and LC-MS/MS chromatographic methods[2-5]. Results showed that both leaves and the seeds of the R. telianum have high inhibitory properties over DPPH radicals with 20.79 µg/mL, r2=0.9884 and 5.67 µg/mL, r2=0.9969 IC50 values, respectively. Similarly, metal-reducing capacities for the extracts were determined as high as standard antioxidants in the CUPRAC, Fe+3, and FRAP assays. The total phenolic and flavonoid content of the leaves and the seeds was determined as directly proportional to the extracts’ antioxidant properties. The samples' dominant secondary metabolites were evaluated from the results obtained from the LC-MS/MS analysis. In conclusion, the structure and functional relationship of the extracts and the possible usage of the plants in the food, medicine, and cosmetic industries were made.