Determination of Flurbiprofen in Human Plasma by Gas Chromatography with Mass Spectrometry and Its Pharmacokinetics
JOURNAL OF AOAC INTERNATIONAL, cilt.97, sa.4, ss.1061-1066, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 97 Sayı: 4
- Basım Tarihi: 2014
- Doi Numarası: 10.5740/jaoacint.12-041
- Dergi Adı: JOURNAL OF AOAC INTERNATIONAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1061-1066
- Atatürk Üniversitesi Adresli: Evet
Özet
This paper describes a GC/MS method for the determination of flurbiprofen in human plasma. Flurbiprofen and internal standard ibuprofen were extracted from plasma by using a liquid-liquid extraction method. Derivatization was carried out using N-Methyl-N-(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10 and 5.0 mu g/mL. Intraday and interday precision values for flurbiprofen in plasma were less than 5.49%, and accuracy (relative error) was better than 5.33%. The extraction recoveries of flurbiprofen from human plasma were between 93.6 and 98.6%. The LOD and LOQ of flurbiprofen were 0.03 and 0.10 mu g/mL, respectively. This assay was applied to determine the pharmacokinetic parameters of flurbiprofen in healthy Turkish volunteers who had been given 100 mg of flurbiprofen.