Akademik Veri Yönetim Sistemi
International Congress on Advances in Biosciences and Biotechnology (ICABB), Sarajevo, Bosna-Hersek, 25 - 29 Ekim 2017, ss.15
Phenazines and derivatives are nitrogen-containing heterocyclic compounds that are
synthesized by Pseudomonas and Streptomyces species. These molecules are redox
agents with broad-spectrum anti-microbial, anti-tumor and anti-parasitic activity.
Over the past decades, there has been great interest in their biotechnological
production because of their unique properties. Bacterial fermentation processes have
advantages over chemical synthesis, since necessity of the highly toxic substrates
and harsh conditions during the chemical synthesis, formation of toxic by-products
and also low productivity. The aim of the study is to produce phenazine-1 carboxylic
acid (PCA) by bacterial fermentation as a bioactive agent. For this purpose, PCA was
produced by P. chlororapsis subsp. aureofaciens on the batch mode in submerged
cultures. Modelling of PCA production and growth of organisms was determined by
temporal analysis of variations of biomass, product, depleted carbon and nitrogen
source. Chromatographic and spectroscopic methods were used for purification,
quantification and characterization of PCA produced by fermentation. Besides, in
vitro anti-cancer and antimicrobial activity of characterized pigment was also
investigated.