Akademik Veri Yönetim Sistemi
2. Uluslararası ve 27. Ulusal Farmakoloji Kongresi / 2nd International and 27th National Pharmacology Congress , Antalya, Türkiye, 23 - 26 Kasım 2023, ss.136-137
Objective: The aim of this study was to investigate the effects of pregabalin, an antiepileptic drug, on wound healing in L929 fibroblast cells. Materyal-Method: Cell Viability Assessment by MTT Method and Scratch Wound Healing Assay L929 cell line obtained from American Type Culture Collection (ATCC, USA) and stored in Cryotube, were removed from the liquid nitrogen tank, and seeded in a T75 cm2 flask containing DMEM medium containing 10% FBS and incubated at 37°C, 90% humidity and 5% CO2. Cells were passaged successively, and cell counting was performed after the fourth passage and 5000 cells were seeded in each well of the 96-well plate. Incubated in an oven with CO2 for 24 hours to allow cells to settle to the bottom of the well. At the end of this period, concentrations of pregabalin (500, 250, 100, 50, 50, 25, 10, 1µM) were prepared and drug cultivation was performed and measured at 24, 48, 72 hours by MTT method with microplate reader spectrophotometer (Epoch Microplate Spectrophotometer, BioTek, USA) at 570 nm absorbance value. Viability rates were analyzed in comparison with control wells. For scratch wound healing assay, L929 cells were seeded in each well of a 6-well plate (2×105 cells/well). After the cells completely covered the bottom of the well, a scratch was made vertically in each well with a sterile 200 µL pipette tip. The wells were then exposed to pregabalin at the concentrations (100, 50, 25, 10 µM) that gave the best results in the cell viability assay. Images of wells were taken at 0, 12, 24 and 36 hours. Quantification of TGF-β1 Production in Vitro Supernatants of all experimental groups were taken at the 36th hour of the experiment. TGF-β1 levels were measured by ELISA method. Results: MTT results showed that pregabalin increased cell proliferation. In the scratch wound healing test results, pregabalin at 100 and 50 µM concentrations significantly increased the percentage of wound closure compared to the control group and other drug groups. When TGF-β1 results were analyzed, it was observed that there was a significant decrease in the TGF-β1 amounts of the drug groups compared to the control group. Conclusion: 100 and 50 µM concentrations of pregabalin have been shown to enhance wound healing. Keywords: wound healing, pregabalin, scratch wound test, L929 cell line