Akademik Veri Yönetim Sistemi
International Black Sea Modern Scientific Research Congress, Rize, Türkiye, 29 Eylül - 02 Ekim 2022
Proteases are an
important group of industrial enzymes and they make up the largest portion of
the entire global enzyme sales. They hydrolyze complex proteins to amino acids
and peptides. Proteases have numerous applications in many industries such as
detergent industry, leather industry, food industry, paper and pulp, silver
recovery from photographic films and bioremediation processes. Although
protease enzymes are widespread in nature and synthesized by plants, animals,
and microorganisms, the preferred protease sources are microbial sources
because they can grow and reproduce quickly and easily, produce enzymes with
high efficiency in simple nutrient media, can be easily manipulated
genetically, can grow in extreme heat, cold, acidic and basic conditions, and
can produce enzymes that can work under these conditions.
The aims of the
present study were isolation, screening, and identification of alkaline
protease producing novel bacteria from water samples of Van Lake. For
prescreening of protease producer bacteria, nutrient agar medium containing 10%
skim milk was used. Among 60 isolates growing at pH 8 and higher, 9 isolates
were chosen as potent alkaline protease producers. These isolates were
identified based on their 16S rDNA sequence analyses, morphological and
biochemical characteristics. Identified strains were further subjected to
quantitative protease activity assay using casein as the substrate. Most potent
isolate was alkaliphilic Exiguobacterium
alkaliphilum strain VLP1 (GenBank Acc No: ON853834.1). Exiguobacterium is a genus of firmicute in the order Bacillales. E. alkaliphilum VLP1 has orange-colored and round colony form with
bacillus cell shape. It is an alkaliphilic, non-motile, Gram (+), catalase (+),
urease (-), hemolysis (-), lipase (-), amilase (-), celulase (-) bacterium.