New Fluoro- and Trifluoromethyl-Substituted Trimethoxychalcones as Anticancer Agents: Synthesis, Bio-evaluation, and Computational Studies

POLAT M. F., ANIL D., YILDIZTEKİN K. G., TÜRKMENOĞLU B., ÖZSOY C., BURMAOĞLU S., ...Daha Fazla

CHEMISTRYSELECT, cilt.8, sa.32, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 32
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/slct.202301189
  • Dergi Adı: CHEMISTRYSELECT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
  • Anahtar Kelimeler: Anticancer activity, chalcones, Claisen-Schmidt condensation, molecular docking, Synthesis, CELL-CYCLE PROGRESSION, CHALCONE DERIVATIVES, APOPTOSIS, ARREST, CANCER, ANTIOXIDANT, FLAVONOIDS, ANALOGS, COMPLEX, METHOXY
  • Atatürk Üniversitesi Adresli: Evet

Özet

We designed and synthesized a novel series of trimethoxy chalcones with CF3 or F substituents at various positions of ring B, characterized using IR, NMR spectral data, and elemental analyses, based on the fact that methoxy and fluoro-substituted chalcones are included in the literature as a pharmacophore due to their anticancer activities. All compounds (12-21) were tested for cytotoxicity against A549, HEPG2, MCF7, and normal mouse fibroblasts (L929) using the XTT assay. The most active compound, 13, was also shown to induce MCF7 cell cycle arrest at the G0/G1 phase, indicating that they exert their antitumor potency via MCF7 cell apoptosis. The mechanisms involved in apoptotic cell death induced by compound 13 were also investigated to see if apoptotic proteins such as Bax, Bcl-2, and p53 were involved. In addition, the compounds with the strongest apoptotic effects against human EGFR and VEGFR-2 receptors were studied in silico. Finally, methoxy and fluoro-substituted chalcones derivatives have been shown to have potent anticancer properties.