Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings

BOZARI S., Agar G., Aksakal O., Erturk F. A., Yanmis D.

TOXICOLOGY AND INDUSTRIAL HEALTH, cilt.29, sa.4, ss.339-348, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1177/0748233711433939
  • Dergi Adı: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.339-348
  • Anahtar Kelimeler: Genotoxic, catmint, GC-MS, RAPD, SDS-PAGE, allelopathy, ANTIBACTERIAL ACTIVITY, METHANOL EXTRACTS, SEED-GERMINATION, MEYERI BENTH., L., ANTIOXIDANT, NEPETALACTONE, BOISS., RAPD
  • Atatürk Üniversitesi Adresli: Evet

Özet

We aimed to determine the genotoxic potential of essential oil (EO) obtained from Nepeta nuda. The chemical content of EO was measured via gas chromatography/mass spectrometry. The most abundant contents were 4a alpha,7 beta,7a alpha-nepetalactone (18.10%), germacrene (15.68%) and elemol (14.38%). For genotoxic effects of EO, Zea mays' seeds were exposed to four different concentrations of this oil. Inhibition of root and stem growth were observed with an increase in EO concentrations. Randomly amplified polymorphic DNA (RAPD) method was used to determine the genotoxic effects of EO. Some changes occurred in RAPD profiles of germinated EO-treated seeds. Even though total soluble protein quantity vary, the data observed from the protein profiles of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that there was a little differentiation between band profiles of treated samples and control group. We concluded that the basis of interactions between plants, like allelopathy, may be related with genotoxic effects of EO.